Evaluation of biofilm formation and associated slime encoding determinants in Staphylococcus aureus isolated from clinically diseased pets

Staphylococcus aureus (S.aureus) is an important zoonotic pathogen implemented in various hospital, community as well livestock infections. Pets dogs and cats have increased their close social relation with human leading to significant elevation transmission of zoonoticmultidrug resistant virulent pathogens.Diplex polymerase chain reaction was applied on ten clinical isolates obtained from wound nasal samples for detection twoslime formation encoding genes;icaA andicaD. The positive genes carrying were encouraged produce biofilm evaluated phenotypically by cultivation onto Congo red medium. Quantitative assessment done using a microtiter plate assay, also the formed examined fluorescent scanning electron microscopy. Four out tested tenStaphylococcus found harbor two separately.The four displayed production medium while only three produced biofilmin sterile polystyrene 96-well plate. One isolate associated dog infection developed strong which byboth microscopy.Staphylococcus diseased can that increasing virulence pathogenicity public health concern.